In order to further broaden the design thought of vehicle suspension applying inerter, two types of vehicle ISD suspension have been designed based on the two parallel elements‘inerter and spring’ by using the anti-resonance of the inerter-spring-mass structure. Using genetic algorithm to get the optimized structural parameters of suspension, analysis in frequency showed that, compared with the passive suspension, the designed suspension structures can suppress the vibration at the offset frequency of the body resonance. Experiment on the second generation suspension has been carried out, a quarter vehicle suspension test bench including the second suspension structure has been built. In the random road input, the suspension working space square mean root has improved by 16.24%, the dynamic tire load square mean root has improved by 6.75%, and the body acceleration square mean root has also improved. The results show that the designed suspension structure can effectively improve ride performance and control stability.