Three dimensional geometrical data of tympanic membrane were got by using sections of undecalcified polymer-embedded temporal bone. An efficient and high precision method using mixed element of the quadrangle element and triangle element was introduced into the mesh of eardrum by HyperMesh software. The meshed model was imported into CAE software ANSYS. After the analysis of statics under different sound pressure, natural vibration and harmonic response, the results were compared with the published data and the reliability based on the mixed element eardrum model was verified. The work in this paper provides theoretic foundation for subsequent research on ossicular chain of middle ear system.