The isolation efficiency of multiple friction pendulum bearings (MFPB) is checked by shaking table test. The results show that changes of load on the isolation layer make no difference in the isolation effect of MFPB and the cooperation of MFPB with viscous dampers can control the displacement of the isolated structure. The test also verify that the vertical seismic input has no significant impact on the isolation effect of MFPB and it is feasible to simulate MFPB by using friction-connection element in SAP2000, with precision which can meet the requirement for application in engineering.