To achieve comprehensive assessment of motorcycle frame fatigue reliability indoor under the impact of moving load in real road, the acquisition method of road load spectrum based on the finite element analysis of the frame was firstly established, and the collection and analysis of the road load spectrum was then completed.A multi-axial,multi-channel road simulation test platform for motorcycle frame was designed and built based on the practical driving load analysis of motorcycle frame,combined with the remote parameter control (RPC) technology , American MTS hydraulic servo actuator and the control system . And the force - displacement mixed control simulation iterative method of road load spectrum was put forward.Combined with the acquired road load spectrum of the motorcycle frame and the mixed loading control mode—force loading control and inertial loading control , road load spectrum of motorcycle frame was efficiently and accurately simulated indoor and a multi axial multi channel road simulation test method of motorcycle frame was established ,providing an effective indoor method for the fatigue reliability test for the motorcycle frame.