By researching the bearing behavior of the variable-stiffness rubber bushing, both the finite element numerical analysis and experimental results show that the stiffness curve has obvious nonlinearity. The stiffness value is small and smooth under normal load conditions, while it grows up significantly with obvious inflection point in the stiffness curve under ultimate load. The position of the inflection point in stiffness curve can be directly affected by changing the height dimension of the stop catch in rubber bushing. Hence the stiffness of the rubber bushing can be changed by adjusting height of stop catch according to working conditions. The analysis results of finite element are used to calculate the equivalent stress range of peril point in the rubber bushing under fatigue loading according to hyperelastic characteristics of rubber material, and fatigue life of the rubber bushing is predicted with S-N curve and then is verified by fatigue bench test result. The result indicates that the rubber bushing is not failed after 1.5 million cycles of fatigue test, and the test fatigue life is accordant with the predicted fatigue life.