Stress-strain curves at quasi-static and high strain rate of 2024-T3 aluminum alloy are measured using dynamic tensile tests by the electronic universal testing machine and split Hopkinson tensile bar (SHTB). Constitutive model of aluminum alloy is described by Johnson-Cook model which can reflect strain and strain rate hardening effect. 4 parameters in Johnson-Cook equation are fitted by the stress-strain curves at different strain rates. By coupling Smooth Particle Hydrodynamics (SPH) method and Finite Element (FE) method, combining with the Johnson-Cook equation of 2024-T3 aluminum alloy, the numerical model of bird impact on a 2024-T3 aluminum alloy square plate is established using the transient dynamic software PAM-CRASH. Dynamic responses of the plate by numerical calculation agree well with the experimental results, which shows that the numerical calculation is reasonable and reliable. The analysis process including material dynamic mechanical test, numerical calculation and bird impact experimental verification provides reference for anti-bird impact design and analysis of aircraft structure.