The simulator separation test-bed and daughter release mechanism are introduced. The phenomenon of oscillation appears in the daughter and maternal separation test. The oscillation source is analyzed in theory by building model. Combined with a large number of experiments, the oscillation source reasons and influencing factors are summarized and put forward for improvement measure. Through analyzing oscillation source, the posture and speed of the daughter and maternal can be measured more accurately in separation process, it provides data basis for real space environment on orbit separation.