The vibration caused by subway has a certain effect on multilayer frame structure and high-rise frame structure. Aimed at the vibration isolation issues of a proposed teaching building located in Guangzhou City, the environmental vibration of the proposed building site was measured. It shows that vertical vibration level of the proposed building site is beyond the limits of codes and higher than horizontal vibration level; In the dynamic response analysis of upper structure, the most unfavorable vibration acceleration time history that we had measured as consistent incentive to the substrate of structure was input. It shows that the vibration of 3.15Hz ~ 31.5Hz is amplified after transmitting to the structure, vertical vibration level of the structure increases layer by layer; The results show that we can effectively reduce the vertical vibration caused by subway by 23% and meet the limits of codes after using steel spring floating vibration isolation in the base of the building.