The effects of local openings on wind loads on enclosed large span spatial structures were considered. Wind loads distribution on enclosed three-center cylindrical latticed shell with local openings is studied via rigid model wind pressure test in wind tunnel, Compare with wind force coefficients and wind loads distribution on structures in different local openings cases, the variation of wind loads distribution with local openings is cleared，such as the suction on structure surface was proved to decrease with the openings in strong negative wind pressure region whereas increase with the openings in positive wind pressure region, and provide suggestion for wind-resistant design and wind-resistant measurements development.