To address the requirement that the sample commands of all the nodes should be triggered synchronously and the synchronization error be less than one sampling period in the course of mechanical vibration synchronous acquisition using Wireless Sensor Net, a WSN Synchronization Trigger protocol based on Beacon Timing Compensation (STBTC_P) is designed. The protocol is developed based on IEEE 802.15.4 Standard, it transmissions acquisition commands with beacon. STBTC_P establishes a good foundation for synchronization acquisition by effectively avoiding the beacon conflict using the centralized associated beacons allocation method first and then achieving trigger synchronous using the beacon timing compensation method for a multi-hop network. Finally, STBTC_P is run on the self-designed acquisition node WSN-G2 and verified its effectiveness.